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Upon exposure of cells to 10(-6)M cCyd, cell viability was reduced to 20% of control, whereas cytocidal activity was not present after 2 hr … Cytidine 5′-triphosphate (CTP) is a pyrimidine nucleoside triphosphate that is analogous to the better-known ATP, but with cytidine as the base instead of adenine. ; Vreken, P.; Kuilenburg, A.B.P. Cytidine triphosphate Names IUPAC name [(2R,3S,4R,5R)-5-(4-amino-2-oxopyrimidin-1-yl)-3,4-dihydroxyoxolan-2-yl]methyl(hydroxy-phosphonooxyphosphoryl)hydrogen phosphate. CTP is a substrate in the synthesis of RNA. Nucleotide structure A nucleotide is comprised of a ring of nitrogen, carbon and oxygen atoms, a five carbon sugar (together referred to as a nucleoside) and one or more phosphate groups. QTY : Add to my cart. Key Words: Acetylcholine-Choline-Cytidine-PC 12 cells-Phosphatidylcholine-Phospholipids. Synthesis of 8-vinyladenosine 5′-di- and 5′-triphosphate: evaluation of the diphosphate compound on ribonucleotide reductase. The major difference between the two molecules is the base used, which in CTP is cytosine. Cytidine: Guanosine: 8-Oxo-2-deoxyguanosine: Wybutosine: Zebularine ★ Inosine triphosphate - nucleosides .. Add an external link to your content for free. The mechanism of action of the cyclopentenyl analogue of cytidine, cCyd, was investigated in human colon carcinoma cell line HT-29. It is used in the synthesis of RNA by RNA polymerases. Reaction mechanism. Cytidine triphosphate is a pyrimidine nucleoside triphosphate. The nucleoside triphosphate pools of two cytidine auxotrophic mutants of Salmonella typhimurium LT-2 were studied under different conditions of pyrimidine starvation. 99% (1) 98% (2) 96% (1) 95% (6) 90 - 94% (1) Physical Form. Cytidine triphosphate is a pyrimidine nucleoside triphosphate. Chemical Synthesis (1) Molecular Biology (2) Stable Isotopes (4) Special Grade. Cytidine 5'-triphosphate (CTP) is a pyrimidine nucleoside triphosphate that is involved in a variety of biochemical reactions. Convenient Syntheses of Cytidine 5'-Triphosphate (CTP), Guanosine 5'-Triphosphate (GTP), and Uridine 5'-Triphosphate (UTP) and Their Use in the Preparation of UDP-Glucose, UDP-Glucuronic Acid, and GDP-Mannose Generally, these enzymes hydrolyze some nucleoside 3',5'-cyclic phosphate to some nucleoside 5'-phosphate thus controlling the cellular levels of the cyclic second messengers and the rates of their degradation. Glazer RI, Knode MC, Lim MI, Marquez VE. The Km for cytidine triphosphate was 0.16 mmole/liter and the apparent Km for phosphatidic acid was 6.2 mmoles/liter. CTP synthetase is the rate-limiting enzyme in the synthesis of cytosine nucleotides, which play an important role in various metabolic processes and provide the precursors necessary for the synthesis of RNA and DNA. It is a substrate in the biosynthesis of RNA. Academic disciplines Business Concepts Crime Culture Economy Education Energy Events Food and drink Geography … Sigma-Aldrich (13) Supelco (1) Manufacturer Name. In the formation of phosphatidylcholine (PC), CTP reacts with phosphocholine, via CTP:phosphocholine cytidylyltransferases, to produce CDP-choline and diphosphate. It is used in the synthesis of RNA by RNA polymerases. Tetrahedron 2003 , 59 (37) , 7315-7322. Search: Add your article Home Carbohydrates Glycosides Glycosides by aglycone type Nucleosides Inosine triphosphate. Triphosphates like CTP and ATP are high-energy molecules; but ATP is involved in many more metabolic reactions than CTP. BP (2) Cytiva (2) EDQM (3) Roche (1) USP (1) Purity. Catalog No.S6077 CAS No. Cytidine triphosphate is a pyrimidine nucleoside triphosphate. In the formation of phosphatidylcholine (PC), CTP reacts with phosphocholine, via CTP:phosphocholine cytidylyltransferases, to produce CDP-choline and diphosphate. Now, however, CTP may be useful in the development of … Cancer cells that exhibit increased cell proliferation also exhibit an increased activity of CTP synthetase. CTP is a high-energy molecule similar to ATP, but its role as an energy coupler is limited to a much smaller subset of metabolic reactions. 36051-68-0 Cytidine 5'-triphosphate is a pyrimidine nucleoside triphosphate that is involved in a variety of biochemical reactions. Cytidine triphosphate synthetase (CTPS) catalyzes the rate-limiting step in the de novo synthesis of CTP, and both the yeast and human enzymes have been reported to be regulated by protein kinase A or protein kinase C phosphorylation. The enzymatic activity could not be dissociated from platelet particles and the greatest specific activity was found in the membrane fraction. CTP acts as an inhibitor of the enzyme aspartate carbamoyltransferase, which is used in pyrimidine biosynthesis. Cytidine triphosphate is a pyrimidine nucleoside triphosphate. It is the rate-limiting enzyme for the synthesis of cytosine nucleotides from both the de novo and uridine salvage pathways.. It is used in the synthesis of RNA by RNA polymerases. diphosphate diglyceride from cytidine triphosphate and phosphatidic acid. Bothmutants,DP45andDP-55,aredefectivein cytidinedeaminaseand cytidine triphosphate (CTP) synthase. An inhibitor of cytidine triphosphate synthesis in human colon carcinoma cells. Convenient Syntheses of Cytidine 5'-Triphosphate, Guanosine 5'-Triphosphate, and Uridine S'-Triphosphate and Their Use in the Preparation of UDP-glucose, UDP-glucuronic Acid, and GDP-mannose Ethan S. Simon,l Sven Grabowski,2 and George M. Whitesides* Department of Chemistry, Haruard Uniuersity, Cambridge, Massachusetts 02138 Receiued September 20, 1989 This paper compares … novel compounds cytidine diphosphate choline and cytidine diphosphate ethanolamine are examples, has been found to play an essential r&e in the biosynthesis of phospholipides. Cytidine deaminase (CDA) is a pyrimidine salvage enzyme that catalyzes cytidine and deoxycytidine hydrolytic deamination to yield uridine and deoxyuridine. CTP (cytidine triphosphate) synthetase catalyzes the last committed step in pyrimidine nucleotide biosynthesis: ATP + UTP + glutamine → ADP + P i + CTP + glutamate . Cytidine triphosphate synthetase (CTPS) is the rate-limiting enzyme in de novo CTP synthesis and is required for the formation of RNA, DNA, and phospholipids. It is a precursor of cytidine triphosphate (CTP) needed in the phosphatidylcholine (PC) and phosphatidylethanolamine (PE) biosynthetic pathways. Exogenous cytidine, acting via cytidine triphosphate, can thus affect the synthesis and levels of cell membrane phospholipids. CTP is a substrate in the synthesis of RNA. Thenucleoside triphosphate pools oftwocytidine auxotrophic mutants ofSal-monella typhimurium LT-2 were studied under different conditions of pyrimidine starvation. Cytidine 5′-triphosphate (disodium salt) For research use only. A subsidiary of Cambridge Isotope Laboratories, Inc. Informations; About Us; Contact Us; Distributors; Custom Synthesis… 2',2'-Difluorodeoxycytidine (dFdC, Gemcitabine) is a deoxycytidine analogue which, after phosphorylation to the 5'-di- and 5'-triphosphate (dFdCTP), induces inhibition of DNA synthesis and cell death. 4NH3: MW: 554,26: Request For Quote. HU also exerts a synergistic effect with nucleoside analogs used in the treatment of human immunodeficiency virus (HIV)-1. Van 1999-01-04 00:00:00 Introduction CTP synthase (EC 6.3.4.2) catalyzes the formation of CTP from uridine triphosphate (UTP) with the concomitant deamination of glutamine to glutamate. These variations probably reflect the species differences in cytidine deaminase, the enzyme that converts cytidine to uridine in the body. The enzyme Cytidine 5’-triphosphate (CTP) synthetase (EC 6.3.4.2) catalyzes a critical reaction in pyrimidine nucleotide biosynthesis as well as phospholipid formation [1-3]. Cytidine triphosphate (CTP) synthetase is a key enzyme for the synthesis of cytosine (deoxy)ribonucleotides, catalysing the conversion of uridine triphosphate (UTP) into CTP, and has a high activity in several malignancies. This paper will describe the enzymatic synthesis of cytidine diphosphate choline and cytidine diphosphate ethanol- Other names CTP; Cytidine-5'-triphosphate; Cytidine 5'-(tetrahydrogen triphosphate) Identifiers CAS Number. Here we report the biochemical characterization of Trypanosoma brucei CDA as an enzyme within the tetrameric class of the CDA family that efficiently deaminates cytidine, deoxycytidine, and the nucleoside analogue 5-methyl-2′-deoxycytidine. Cytidine triphosphate (CTP) synthase blockade by CPEC leads to inhibition of smooth muscle cell (SMC) proliferation. The T7 RNA polymerase synthesis of modified RNA can be very efficiently used for synthesis of modified RNA but the method has constraints in the sequence of the first three nucleotides of the transcript, which must contain a non-modified G in the +1 position. 13 ) Supelco ( cytidine triphosphate synthesis ) Manufacturer Name salvage pathway to synthesize CTP using cytidine,,. Aspartate carbamoyltransferase, which in CTP is a pyrimidine nucleoside triphosphate that involved... 4 ) Special Grade 5′-triphosphate ( disodium salt ) for research use only uridine triphosphate pyrimidine biosynthesis a! Pathway to synthesize CTP using cytidine, cCyd, was investigated in human colon carcinoma cytidine triphosphate synthesis line HT-29 uridine... Variety of biochemical reactions DP45andDP-55, aredefectivein cytidinedeaminaseand cytidine triphosphate ( CTP ) is a substrate in the fraction... 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